Journal: JCI Insight
Article Title: Matrix metalloproteinases are hallmark early biomarkers and therapeutic targets in FSHD
doi: 10.1172/jci.insight.195104
Figure Lengend Snippet: ( A ) UMAP plot of merged single-cell data from uninduced WT (control) and dox-induced iDUX4 mice (10 days on 625 mg/kg dox chow), with clusters annotated by cell types. ( B ) UMAP comparison of cell clusters between control and DUX4-induced muscles. ( C ) Proportional representation of major cell populations in control and DUX4-induced muscles. ( D and E ) UMAP plots showing the spatial distribution and cellular origin of MMP expression in control ( D ) and DUX4-induced ( E ) muscles. ( F ) Bubble plots highlighting distinct MMP expression patterns across cell types. ( G ) Normalized fold-change in MMP expression within selected cell populations. ( H ) Violin plots showing expression levels of Mmp2 , Mmp14 , and Mmp19 across annotated cell clusters. Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 by Student’s 2-tailed t test. ECs, endothelial cells; MSCs, mesenchymal stem cells; SCs, satellite cells; UMAP, uniform manifold approximation and projection.
Article Snippet: For immunofluorescence, tissue sections or sorted FAPs were fixed in 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 for 30 minutes, and incubated overnight at 4°C with primary antibody against collagen VI (1:200, Proteintech), MMP2 (1:500, Proteintech), MMP14 (1:500, Proteintech), PDGFRα (1:200, BioLegend), F4/80 (1:200, BioLegend), and Ki-67 (1:400, BioLegend), followed by secondary antibody conjugate to Alexa Fluor 488, 555, or 647 (1:500, Thermo Fisher Scientific) for 2 hours at room temperature.
Techniques: Control, Comparison, Muscles, Expressing
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